![]() An integrated physical and genetic map of the nematode Pristionchus pacificus. A physical amplified fragment-length polymorphism map of Arabidopsis. TOPAAS, a tomato and potato assembly assistance system for selection and finishing of bacterial artificial chromosomes. Further characterization of AFLP data as a tool in genetic diversity assessments among maize ( Zea mays L.) inbred lines. Vuylsteke, M., Mank, R., Brugmans, B., Stam, P. Wageningen University, Wageningen, The Netherlands., ISBN 90-5808-044-7 (1999). Genetic Analysis of Maize by Using the AFLP Method. Chromosomal regions involved in hybrid performance and heterosis: their AFLP-based identification and practical use in prediction models. An association study using AFLP markers and application to a beef cattle breeding population. Comparison of linkage disequilibrium in elite European maize inbred lines using AFLP and SSR markers. Linkage disequilibrium mapping of the bolting gene in sea beet using AFLP markers. Hansen, M., Kraft, T., Ganestam, S., Sall, T. The establishment of 'essential derivation' among rose varieties, using AFLP. Vosman, B., Visser, D., van der Voort, J.R., Smulders, M.J. Estimating genetic conformity between related ryegrass (Lolium) varieties. Evaluation of the DNA fingerprinting method AFLP as an new tool in bacterial taxonomy. Site of einkorn wheat domestication identified by DNA fingerprinting. AFLP: a new technique for DNA fingerprinting. This latter feature has been exploited for co-dominant scoring of AFLP markers in sample collections such as F 2 or back-cross populations using appropriate AFLP scoring software. Crucial characteristics of the AFLP technology are its robustness, reliability and quantitative nature. AFLP fragments may also be used as physical markers to determine the overlap and positions of genomic clones and to integrate genetic and physical maps. The AFLP technology has been used predominantly for assessing the degree of variability among plant cultivars, establishing linkage groups in crosses and saturating genomic regions with markers for gene landing efforts. Visualization of AFLP fingerprints after gel electrophoresis of AFLP products is described using either a conventional autoradiography platform or an automated LI-COR system. ![]() Two limited sets of AFLP primers are sufficient to generate a large number of different primer combinations (PCs), each of which will yield unique fingerprints. The protocol involves the selective PCR amplification of restriction fragments of a total digest of genomic DNA, typically obtained with a mix of two restriction enzymes. The AFLP technique is a powerful DNA fingerprinting technology applicable to any organism without the need for prior sequence knowledge. ![]()
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